Figure 4. 2DG inhibits autophagic flux. Differentiated SH-SY5Y cells were treated with 20 mM 2DG for 24 h, followed by 30 μM HNE in the presence or absence of 40 μM chloroquine (CQ) for 2 h. (A) Western blot analyses of protein extracts from control or 2DG- and HNE-treated cells in the presence or absence of 40 μM CQ for MAP1LC3A-I and MAP1LC3A-II. (B) Quantification of MAP1LC3A-II from (A). Data represent mean ± SEM (n = 3), normalized to HNE+CQ. *P < 0.05, the Student t test. (C) Western blot analyses of protein extracts from control or 2DG- and HNE-treated cells for SQSTM1. (D) Quantification of SQSTM1 from (C). Data represent mean ± SEM (n = 3), normalized to 2DG. *P < 0.05, the Student t test.