Figure 4. Disrupted emerin localization in cells expressing LAΔNLS or PGΔNLS. (A) Confocal fluorescence images. HeLa cells transiently expressing EGFP-LA, EGFP-PG, EGFP-LAΔNLS or EGFP-PGΔNLS (green) were fixed and stained with anti-emerin (red) at 24 h post transfection. A representative cell under each condition is shown. Scale bar, 10 μm. (B) IP with GFP-Trap®_A beads in the transfected HeLa cells. Un-transfected HeLa cells were used as a control (CT). (C) Relative intensity of emerin to EGFP-tagged proteins in each immunopricipitated sample. Band intensities were analyzed using ImageJ. Relative intensities were presented as the ratio of emerin to EGFP. Two biological duplicates were conducted. A representative experiment was shown.