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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: J Neurochem. 2014 Feb 12;129(5):850–863. doi: 10.1111/jnc.12655

Figure 2.

Figure 2

E2F1 is predominantly cytoplasmic in vivo and in vitro. A) Coronal hippocampal sections from WT P365+ mice were immunolabeled with E2F1 in green and neuronal dendritic marker MAP2 in red (Top). Higher magnification images of the inset areas reveal E2F1 staining can be punctated where neuritic processes are abundant and the cell bodies are absent (Bottom). B) Primary hippocampal neurons at 14 DIV were labeled with E2F1 in green, MAP2 in red, and the nuclei counterstained with DAPI in blue. Two specific E2F1 antibodies KH95 (Top) and KH20 (Middle) were used and produced similar staining patterns. Condition with no E2F1 primary antibodies was included as negative control (Bottom). C) Exogenous E2F1 expression is predominantly cytoplasmic when overexpressed in primary hippocampal neurons. Primary hippocampal neurons were transfected with E2F1 plasmid at 10DIV, fixed at 14 DIV and labeled with E2F1 in green and MAP2 in red. D) Primary cortical neurons at 21 DIV were fixed and labeled with E2F1 in green, MAP2 in red, and nuclei counterstained with DAPI in blue. Scale bar=30μm.