Table 1.
Brain Area | # of Sections |
Counting Frame (in µm) |
Grid (in µm) |
Paxinos & Watson Plate # |
---|---|---|---|---|
Olfactory bulb | 6 | 30 × 30 | 43 × 43 | 1 |
Amygdala | 4 | 50 × 50 | 71 × 71 | 27 – 33 |
Hippocampus | 4 | 30 × 30 | 43 × 43 | 29 – 35 |
Note: The average mounted section thickness was about 22 µm and the optical dissector height was set to 15 µm. The average Gunderson coefficient of error was less than 0.2 across all brain regions.