Figure 1.

Comparison of DPP4 inhibitor-treated donor mouse BM cells and DPP4 inhibitor treated recipient mice on engraftment in the setting of competitive repopulation using congenic mice. Mouse C57Bl/6 (CD45.2⁺) BM cells were treated with Diprotin A (5mM/10⁶ cells) or with control diluent for 1 hour at room temperature prior to washing the cells 2X and mixing the donor cells at a 1:1 ratio with untreated B6.BoyJ competitor (CD45.2⁺) BM cells prior to infusing the donor and competitor cells i.v. at 2×10⁵ cells each into lethally-irradiated (950cGy gamma radiation) dual CD45.2⁺/CD45.1⁺ (F1) recipient mice. Cells were infused 24 hours later into F1 mice that were orally gavaged with control diluent or with 200 μg sitagliptin/mouse 4 hours after radiation. Results are shown as mean ±1SEM for 5 mice per group each assayed individually. ^a, significantly different from controls (control treated donor cells into non-sitagliptin treated recipients), p<0.001.