Figure 5.
IL-1β-mediated neuroinflammation in the DR results in broad changes in gene expression. A, Heat map illustrating significant differences in gene expression between AAV-GFP-infused and AAV-Cre-infused male and female DR RNA from IL-1βXAT mice, where data are expressed as relative levels within each gene, and both animals and genes are organized by hierarchical clustering. B, C, Genes that were either significantly upregulated (B) or significantly downregulated (C), as determined by an false discovery rate of 0.01, are ranked by enrichment score and organized by functional gene clusters identified using the Database for Annotation, Visualization, and Integrated Discovery bioinformatics database (DAVID). D, A separate analysis was performed on genes annotated with the gene ontology term (GO) “neur*,” which identified 26 genes that are downregulated in IL-1β animals infused with AAV-Cre.