Table 3.
Kinetic parameters of M1–M2 linker mutants of the α1 GlyRa
| Mutation | τdeactivation (ms) | τentry into desensitization (ms) | τrecovery from desensitization (s) |
|---|---|---|---|
| Wild type | 3.95 ± 0.48 (0.544 ± 0.036) | 4.92 ± 1.08 (0.448 ± 0.044) | 2.80 ± 0.27 |
| 28.8 ± 3.5 | 100 ± 22 (0.274 ± 0.045) | ||
| (21/29) | 1,380 ± 420 (0.278 ± 0.030) | ||
| (15/38) | |||
| N–7′A | 4.17 ± 1.37 (0.475 ± 0.079) | 4.92 ± 2.73 (0.353 ± 0.098) | 1.90 ± 0.17 (0.845 ± 0.072) |
| 21.8 ± 5.5 | 45.3 ± 13.1 (0.337 ± 0.133) | 13.5 ± 7.6 | |
| (6/6) | 609 ± 165 (0.310 ± 0.090) | ||
| (4/6) | |||
| N–7′T | 3.09 ± 0.68 (0.689 ± 0.061) | 3.59 ± 0.83 (0.298 ± 0.055) | 1.42 ± 0.23 (0.748 ± 0.148) |
| 12.8 ± 2.6 | 43.4 ± 18.9 (0.293 ± 0.090) | 5.98 ± 2.53 | |
| (6/6) | 1,140 ± 470 (0.409 ± 0.096) | ||
| (5/7) | |||
| M–6′A | 4.96 ± 0.26 (0.701 ± 0.035) | 4.98 ± 1.21 (0.248 ± 0.042) | 6.41 ± 2.40 |
| 15.2 ± 1.3 | 54.2 ± 14.9 (0.336 ± 0.084) | ||
| (3/3) | 962 ± 299 (0.416 ± 0.082) | ||
| (6/7) | |||
| M–6′T | 1.30 ± 0.37 (0.596 ± 0.193) | 3.30 ± 2.44 (0.239 ± 0.117) | 1.15 ± 0.16 (0.692 ± 0.100) |
| 15.7 ± 5.9 | 33.3 ± 3.9 (0.172 ± 0.005) | 5.94 ± 1.57 | |
| (3/4) | 369 ± 77 (0.589 ± 0.112) | ||
| (2/5) | |||
| D–5′A | 3.35 ± 0.49 (0.614 ± 0.037) | 21.7 ± 10.8 (0.491 ± 0.113) | 2.58 ± 0.30 |
| 12.1 ± 1.6 | 265 ± 128 | ||
| (3/6) | (3/6) | ||
| D–5′T | 8.81 ± 3.58 (0.613 ± 0.126) | 17.5 ± 10.5 (0.193 ± 0.036) | 1.71 ± 0.14 |
| 40.7 ± 16.2 | 238 ± 30 (0.382 ± 0.026) | ||
| (4/5) | 878 ± 8 (0.425 ± 0.048) | ||
| (3/6) | |||
| A–4′T | 2.77 ± 0.90 (0.581 ± 0.065) | 6.25 ± 2.5 (0.194 ± 0.062) | 0.102 ± 0.012 (0.518 ± 0.026) |
| 9.78 ± 1.86 | 69.1 ± 20.1 (0.306 ± 0.117) | 3.62 ± 0.46 | |
| (6/6) | 1,440 ± 750 (0.500 ± 0.081) | ||
| (3/6) | |||
| A–3′T | 12.8 ± 3.0 (0.255 ± 0.072) | 3.42 ± 0.56 (0.346 ± 0.100) | 0.114 ± 0.020 (0.495 ± 0.046) |
| 50.5 ± 4.1 | 36.7 ± 6.1 (0.148 ± 0.026) | 2.40 ± 0.47 | |
| (6/9) | 3,840 ± 2,600 (0.506 ± 0.079) | ||
| (4/8) | |||
| P–2′A | 3.70 ± 0.52 (0.307 ± 0.042) | 59.0 ± 20.6 (0.247 ± 0.035) | 0.247 ± 0.052 (0.441 ± 0.040) |
| 35.7 ± 6.7 | 6,010 ± 1,430 | 6.52 ± 0.83 | |
| (5/7) | (6/9) |
aAll errors are SEs. The fractions in parentheses have the same meaning as in Table 2. Each time course was obtained from a different patch of membrane.