Figure 10.

Analysis of TTR expression in SH-SY5Y, HepG2 cells, and WT C57BL/6 embryonic hippocampal neurons transiently expressing human HSF1 or shHSF1 plasmids. qRT-PCR of TTR mRNA (relative to endogenous actin mRNA) extracted from SH-SY5Y (A) and HepG2 (B) cells transfected with pcDNA empty vector, scrambled shRNA, a GFP construct serving as a marker of transfection efficiency (∼20%, i.e., GFP positive cells vs total cells) and an HSF1 construct, SH-SY5Y (A) and HepG2 (B). The HSF1 construct significantly induces TTR mRNA in neuronal cells over that seen with any of the controls in the SH-SY5Y cells. Only the antisense HSF1 construct has any effect in the HepG2 cells (B). Quantitation of hHSF1 (C) or mTtr, hsp70, hsp90 mRNAs (D) in cultured primary neurons transfected with either pcDNA empty vector, GFP, or HSF1 (n = 20 embryos from 4 dams). Error bars indicate mean ± SD. Statistical significance is indicated (from ≥3 independent experiments, Student's t test): **p < 0.01.