Figure 14.

Ttr expression and HSF1 binding to the Ttr promoter in APP23 transgenic mice. A, Ttr mRNA was analyzed in hippocampal cells from WT (n = 4), APP23 (n = 6), and APP23/mttr^−/− (n = 3) mice. B, Quantitation of Ttr mRNA in hepatic cells from WT (B6), APP23, and APP23/mttr^−/−. C, HSF1 binding to the Ttr promoter in the hippocampus of APP23 mice (n = 6) compared with WT mice (n = 4) by ChIP assay. D, ChIP assays of HSF1 binding to Ttr promoter in hepatic cells of APP23 (n = 6) and WT mice (n = 4). The controls include input DNA before immunoprecipitation and a normal mouse IgG precipitation. Error bars indicate mean ± SD. Statistical significance is indicated compared with WT mice: *p < 0.05; **p < 0.01.