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. 2014 May 21;34(21):7253–7265. doi: 10.1523/JNEUROSCI.4936-13.2014

Figure 5.

Figure 5.

Effect of heat shock on Hsp and TTR expression in SH-SY5Y and HepG2 cells. SH-SY5Y (A) and HepG2 cells (B) were incubated at 42°C for the indicated periods. Samples were harvested for RNA isolation and analyzed by qRT-PCR analysis. A, Heat shock increases Hsp40 (black), Hsp70 (gray), Hsp90 (dark gray), and TTR (white) mRNA levels in SH-SY5Y cells compared with untreated (NT) cells. B, Heat shock increases Hsp mRNA expression, but TTR mRNA levels do not change in HepG2 cells compared with the NT cells. Error bars indicate mean ± SD. Statistical significance of differences between mRNA levels of treated and untreated cells (NT) is indicated (from ≥3 independent experiments, Student's t test): *p < 0.05; **p < 0.01. Protein extracts from heat shock-treated SH-SY5Y (C) or HepG2 (D) cells probed with antibodies to heat shock proteins.