Figure 3.

Set-β expression is not associated with generalized cellular toxicity. A, Acutely purified P4 RGCs transfected with mCherry or wild-type Set-β were immunostained at 1, 2, and 3 d for reporter tag (red), Tuj1 (neurite marker, green), and counterstained with DAPI (nuclear marker, blue). Nuclei outlined with white dashed line. Scale bar, 20 μm. B, At 1, 2, and 3 d, the number of RGCs transfected with constructs as marked were counted per unit area normalized to 1 d (106 cells per condition); no significant difference between the conditions was observed (mean ± 95% CI shown; nonsignificant by ANOVA with repeated measures, with post hoc LSD). N.S., Not significant.