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. Author manuscript; available in PMC: 2015 Jun 28.
Published in final edited form as: Cancer Lett. 2014 Mar 21;348(0):156–166. doi: 10.1016/j.canlet.2014.03.016

Figure 3. Triptolide induced decrease in c-FLIP expression sensitizes pancreatic cancer cells to TRAIL mediated cell death.

Figure 3

A. c-FLIP expression was detected by western blot MIA PaCa-2 and S2-VP10 cells treated with TRAIL or triptolide or a combination of both for 24h. β-actin expression was used as loading control.

B. Knockdown of c-FLIP by siRNA in MIA PaCa-2 and S2-VP10 cells was confirmed by western blot. β-actin expression was used as loading control.

C. Treatment of MIA PaCa-2 and S2-VP10 cells with a combination of TRAIL and c-FLIP siRNA significantly decreases cell viability after 24 hours of treatment. The bars represent mean ± SEM, n≥3. **p< 0.01.

D. PARP and caspase-3 cleavage was assessed in MIA PaCa-2 and S2-VP10 cells after treatment with TRAIL or c-FLIP siRNA or both using western blot. β-actin expression was used as loading control.

E. Treatment of MIA PaCa-2 and S2-VP10 cells with TRAIL or c-FLIP siRNA or a combination of both shows a significant increase in caspase-8 activation 24 hours post treatment. The bars represent mean ± SEM, n≥3. **p< 0.01.