Skip to main content
. 2014 Apr 5;16(4):e14914. doi: 10.5812/ircmj.14914

Figure 1. High Glucose Exposure Resulted in Translocation of FOXO3a to Nuclei.

Figure 1.

NRVMs were incubated in serum free DMEM treated with either normal glucose (5 mM) or high glucose (30 mM) for 24 hours. The expression level of total FOXO3a as well as nuclear FOXO3a was examined by subcellular fractionation followed by Western blotting and then the densitometry was analyzed quantitatively. The expression of total FOXO3a and nuclear FOXO3a were normalized to loading control GAPDH. Data represent mean ± SD (n = 3). ** P < 0.01 versus respective normal glucose controls.