Table 2.
Detection of EHV-1 gB DNA by nested gB PCR in ocular and nervous system tissues collected post mortem following infection of ponies with Ab4∆75-LacZ (experiment 1).
|
Pony # |
A |
B |
C |
D |
E |
F |
G |
H |
|
|---|---|---|---|---|---|---|---|---|---|
| Tissue | Day pi/euthanasia | 1 | 2 | 3 | 5 | 9 | 12 | 19 | 23 |
| Cornea |
+ |
+ |
- |
+ |
- |
+ |
+ |
- |
|
| Aqueous |
- |
+ |
+ |
- |
+ |
- |
- |
- |
|
| Vitreous |
- |
- |
- |
+ |
+ |
+ |
- |
- |
|
| Iris |
- |
- |
- |
- |
+ |
- |
- |
- |
|
| Choroid |
- |
- |
- |
- |
- |
+ |
- |
- |
|
| Retina |
- |
- |
- |
- |
+ |
+ |
+ |
+ |
|
| Optic nerve |
- |
- |
- |
- |
+ |
- |
+ |
+ |
|
| Olfactory lobe |
- |
- |
- |
- |
+ |
|
+ |
- |
|
| Cerebrum (frontal) |
- |
- |
- |
- |
+ |
- |
- |
+ |
|
| Cerebrum (parietal) |
- |
- |
- |
+ |
- |
- |
+ |
- |
|
| Cerebrum (occipital) |
- |
- |
- |
- |
+ |
- |
- |
- |
|
| Cerebrum (temporal) |
- |
- |
- |
- |
- |
+ |
+ |
- |
|
| Choroid plexus |
- |
- |
- |
+ |
+ |
+ |
- |
+ |
|
| Diencephalon |
- |
- |
- |
- |
+ |
- |
+ |
+ |
|
| Midbrain |
- |
- |
+ |
- |
+ |
+ |
- |
- |
|
| Pons |
- |
- |
- |
- |
+ |
+ |
- |
+ |
|
| Medulla |
- |
- |
- |
- |
+ |
+ |
- |
+ |
|
| Cerebellum |
- |
- |
- |
- |
- |
+ |
- |
- |
|
| Trigeminal ganglion | + | + | + | + | + | + | + | + | |
+ detection of EHV-1 gB DNA; - no detection of EHV-1 gB DNA.