Table 5.
Intrastadial transmission Experiment 1, adult ticks were acquisition fed on splenectomized horse Ho-226 for 4 days; parasitemia averaged 6.21 × 10 7 genome copies per ml of blood over the acquisition feeding period, as determined by qPCR
| Horse # | Tick species | Sex | Incubation (days) a | Transmission feed (days) | # recovered/# applied | # SG pos/# tested | # pos eggs/# ovip b |
|---|---|---|---|---|---|---|---|
| Ho-220 |
A. caj |
♂♂ |
7 |
7 |
47/76 |
0/46 |
|
| Ho-216 |
|
♀♀ |
7 |
7-10c |
33/78 |
0/7 |
0/26 |
| Ho-214 |
D. var |
♂♂ |
11 |
7 |
98/100 |
0/98 |
|
| Ho-219 | ♀♀ | 11 | 6-9c | 97/100 | 0/11 | 0/86 |
After acquisition feeding ticks were placed on 4 different hosts for transmission feeding. None of the transmission hosts acquired infection. After transmission feeding all males and a sample of females were dissected and their salivary glands tested for infection by nPCR. All remaining females that had fed to repletion were held for oviposition and their eggs tested for presence of T. equi DNA.
aTime between acquisition feed and transmission feed.
bNumber of ticks laying positive egg masses/ total number of egg masses tested.
cReflects the amount of time required for female ticks to feed to repletion and drop off.