Table 8.
LongSAGE tags with >10-fold differential expression
| Tag a |
Control |
Infected |
Difference |
BmiGI match |
|||
|---|---|---|---|---|---|---|---|
| Count b | Count b | (fold) | Description | Species | e-value | ||
| Upregulated with infection |
|
|
|
|
|
|
|
| TGATTGTGTGCTATGTG |
1 |
148 |
148 |
Noc |
|
|
|
| GGGGCAAACACTATGGA |
0 |
89 |
>89 |
No |
|
|
|
| AAGATCACACTGGCATT |
1 |
59 |
59 |
No |
|
|
|
| TTTTCCCCAACCCAGGA |
0 |
52 |
>52 |
BEACP61TR |
Microplusin |
R. microplus |
7e-49 |
| TTTTTCCCAACCCAGGA |
1 |
41 |
41 |
No |
|
|
|
| GGTCAAGGGGTAATAAA |
0 |
38 |
>38 |
No |
|
|
|
| GTGGTTACGGAGGCGGG |
0 |
38 |
>38 |
No |
|
|
|
| CAGAAGCTTCAAAGCCA |
1 |
35 |
35 |
No |
|
|
|
| TCGACAGGGTCATTCCG |
0 |
32 |
>32 |
No |
|
|
|
| GCCGTTCTTAGTTGGTG |
1 |
30 |
30 |
No |
|
|
|
| GCGGTTACGGAAGCGGG |
0 |
27 |
>27 |
No |
|
|
|
| AAGATCACGCTGGCATT |
0 |
25 |
>25 |
No |
|
|
|
| CACATCATAGAACAGCT |
0 |
23 |
>23 |
No |
|
|
|
| ACAAATAAAATTGAGCT |
0 |
23 |
>23 |
No |
|
|
|
| TACTGTACCGAGGCCAG |
0 |
18 |
>18 |
No |
|
|
|
| GTTGTTACGGGTAACGG |
0 |
18 |
>18 |
BEAE880TF |
Secreted protein |
I. scapularis |
6e-20 |
| ATATTGACATTTCGTAG |
0 |
18 |
>18 |
TC12054 |
Mitochondrial DNA |
R. sanguineus |
0.0 |
| CCGGTTCTTTCTTGGTG |
0 |
15 |
>15 |
No |
|
|
|
| AAAAAGGCTCAAGAAAT |
1 |
15 |
15 |
No |
|
|
|
| ATCTGAGTTTAGACCGA |
25 |
354 |
14.2 |
TC5761 |
Mitochondrial DNA |
R. sanguineus |
0.0 |
| GGTCAGTCGGTCCTTAG |
1 |
14 |
14 |
TC12507 |
10kD secreted protein |
A. monolakensis, |
5e-28 |
| GTAGCCGCCAGCCAAGG |
2 |
23 |
11.5 |
No |
|
|
|
| CAGGCTGTCCCAGCAAT |
4 |
45 |
11.2 |
BEAE009TR |
Secreted salivary peptide |
I. scapularis |
1e-05 |
| CTTACTGCCCCAGCAAT |
2 |
21 |
10.5 |
TC8005 |
Secreted salivary peptide |
I. scapularis |
9e-15 |
| Down-regulated with infection |
|
|
|
|
|
|
|
| TCTGGACGCGGCAAGGG |
48 |
1 |
-48.0 |
No |
|
|
|
| CACGTACAACCTCTGCG |
22 |
0 |
<-14.0 |
No |
|
|
|
| TTGTGCAGCGATCGGCA |
22 |
1 |
-22.0 |
No |
|
|
|
| GTACCAGAGGACAAGCC |
18 |
1 |
-18.0 |
No |
|
|
|
| TCGAACCCCCGGCAGTA |
14 |
0 |
<-14.0 |
No |
|
|
|
| TGGGGCACGTCCAAGCT |
14 |
1 |
-14.0 |
TC6102 |
Elongation factor beta |
Ornithodoros parkeri, |
1e-85 |
| GTGGTGCCATCGGCGCT |
14 |
1 |
-14.0 |
No |
|
|
|
| TCGAACCCCCGGCAGTA |
12 |
1 |
-12.0 |
TC14104 |
Ribosomal protein S18 |
Ornithodoros parkeri, |
1e-67 |
| GAAGAAGCCATCGGCCG |
12 |
1 |
-12.0 |
No |
|
|
|
| AACCCCGTCGAGCACCC |
12 |
1 |
-12.0 |
TC12306 |
Ribosomal protein L8 |
Glossina morsitans morsitans |
2e-116 |
| GGCCGCTACCCGGACCT |
12 |
1 |
-12.0 |
TC10648 |
Hypothetical protein |
I. scapularis |
1e-93 |
| TGGTGCCCGAAACGAAG |
68 |
6 |
-11.3 |
TC12322 |
Uro-adherence factor A |
Taloromyces stipitatus |
1e-05 |
| AAGAGCGTGTGCGGCTG | 36 | 3 | -11.0 | TC9377 | Hypothetical protein | I. scapularis | 1e-20 |
a CATG trimmed from each tag’s 5′ end for clarity.
b To allow comparisons, tag count data normalized to 8,750 total tag counts per library and individual counts adjusted accordingly. Thus, Control Library tags multiplied by 8750/6764 and Infected Library tags by 8750/8750.
c Indicates no match to BmiGI