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. Author manuscript; available in PMC: 2014 May 21.
Published in final edited form as: Nature. 2010 Sep 2;467(7311):91–94. doi: 10.1038/nature09334

Figure 2. Spindle-independent cleavage furrow positioning.

Figure 2

(a) Sas-4 mutant neuroblast lacks astral microtubules, yet still establishes basal Myosin localization and basal furrow position (100%, n=158).

(b) Colcemid-treated rod mutant neuroblast lacks all spindle microtubules, yet still establishes basal Myosin localization and basal furrow position (100%, n=7).

(c) Colcemid-treated wild type neuroblast lacks all spindle microtubules, remains arrested at metaphase, and does not establish basal Myosin localization or initiate furrow formation (100%, n=7).

A schematic of each experiment is shown to the left (apical/basal polarity, blue/red; microtubules, gray). All genotypes imaged in late second/early third larval instar brains.

Scale bars: 10μm. Time in min:sec.