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. 2013 Nov 5;4(6):136. doi: 10.1186/scrt347

Figure 3.

Figure 3

Elimination and restoration of endogenous primordial germ cells in embryonic gonads by busulfan treatment at stage X. Immunostaining was performed to detect germ cells in 5.5-day-old and 9-day-old embryonic gonads using an anti-VASA primary antibody and PE-conjugated secondary antibody. Scale bar = 100 μm.