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. 2013 Nov 5;4(6):136. doi: 10.1186/scrt347

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Copyright © 2013 Lee et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Proportion of primordial germ cells (PGCs) in the embryonic gonads after busulfan treatment at stage X. (A) Representative flow cytometry dot plots of day 5.5, day 7, day 9 and day 15 embryonic gonadal cells labeled with anti-VASA. (B) Proportion of PGCs in the embryonic gonads of the busulfan-treated group. Data were normalized to the proportion of PGCs in the control. Bars indicate standard error of the mean of triplicate analyses. ***P <0.001, significant difference compared with control.