Figure 5.

Cell cycle analysis in primordial germ cells of 9-day-old embryonic gonads after busulfan treatment at stage X. VASA-positive cell populations in embryonic gonads derived from flow cytometry were evaluated in terms of cell cycle phase. (A) Results of representative cell cycle evaluations in males and females. (B) Results of replicate cell cycle evaluations in males and females. Bars represent the standard error of the mean of triplicate analyses. *P <0.05, significant difference compared with control.