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. 2014 May 21;4:5018. doi: 10.1038/srep05018

Figure 2. Development of AtMYB111-expressing transgenic tobacco lines and their phenotypic characterization.

Figure 2

(a) Schematic representation of T-DNA region of plant expression construct carrying AtMYB111 cDNA in pBI121 vector used for tobacco transformation. (b) Flower color alterations in different transgenic lines. The pigmentation in petals of transgenic lines expressing AtMYB111 (1, 2 and 3) was compared with WT and empty vector (EV) transformed tobacco lines. (c) Confirmation of the presence of the transgene by PCR amplification of transgene using forward and reverse primers of AtMYB111 in different transgenic lines. (d) Expression analysis of transgene through semiquantitative RT-PCR using total RNA from leaves of WT, EV and different transgenic lines. (e) Total anthocyanin content in petals of WT, EV and different transgenic lines. Data are expressed as means ± SE of at least 3 independent experiments, each experiment consisting of 3 technical replicates. In (c) and (d), gels have been cropped for clarity and conciseness of the presentation. *** indicate values that differ significantly from WT at P < 0.001, according to Student's paired t-test.