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. 2014 May 21;4:5018. doi: 10.1038/srep05018

Figure 6. Modulation of flavonoid biosynthesis by light in transgenic tobacco.

Figure 6

(a) Effect of light over the expression of the structural genes involved in flavonoid pathway in the different transgenic lines expressing AtMYB111. Expression of structural genes of phenylpropanoid pathway/flavonoid pathway was analyzed by real time PCR using RNA from the leaves of different transgenic lines expressing AtMYB111. (b) Effect of light over flavonoid biosynthesis in different transgenic lines expressing AtMYB111. Phytochemical analysis of methanolic extracts of leaves in different transgenic lines expressing AtMYB111. Compounds were quantified by separating methanolic extracts from the young leaves of transgenic lines using HPLC. The graph shows values ± SD of three leaves from each of the independent the transgenic line expressing AtMYB111. Data are expressed as means ± SE of at least 3 independent experiments, each experiment consisting of 3 technical replicates. *** indicate values that differ significantly from WT at P < 0.001, according to Student's paired t-test.