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. 2014 Feb 28;15:168. doi: 10.1186/1471-2164-15-168

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Copyright © 2014 Ma et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sperm genes are downregulated by endo-siRNA pathway. (a) Heat-map showing the transcripts in the sperm transcriptome are targeted by antisense 19G-28G primary siRNAs. Each row represents a sperm transcript. Abundance of a sperm transcript is quantified by log₂RPKM. The sperm transcript targeted by the corresponding siRNAs is indicated by a black line. (b) RT-PCR identification of 32 sperm genes that are expressed at higher levels in the rrf-3;him-5, as compared with the him-5.