Skip to main content
. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: Dev Biol. 2013 Dec 27;386(2):408–418. doi: 10.1016/j.ydbio.2013.12.013

Fig. 4. Ecdysone reporter expression is reduced proportional to increasing numbers of mutant cells within the follicular epithelium.

Fig. 4

(A–D) Stage 10 PhmFT59 mosaic egg chambers containing the EcR-LacZ reporter. Egg chambers are stained with Hoechst to label nuclei, and anti-β-galactosidase to detect ecdysone reporter expression. Homozygous mutant cells are marked by the absence of GFP. (A’–D’) show the GFP expression, and (A”–D”) show anti-β-galactosidase staining. Border cells are indicated with an arrow (A”–D”). Egg chambers with an entirely wild-type epithelium exhibit strong expression of the ecdysone activity reporter within the border cells (A”). When larger portions of the cells within the follicular epithelium are mutant, EcR-LacZ reporter expression in the border cells diminishes in direct relation to increasing numbers of mutant epithelial cells (B”–D”). Egg chambers with specific fractions of mutant cells within the follicular epithelium were scored for relative intensity of EcR-LacZ expression within the border cell cluster (F), re-affirming the trend demonstrated in (A”ȓD”).