(A–C) In vitro bioactivities against purified enzyme recorded for BmcA and BmcB against the NIS synthetase (A) SbnE, (B) AsbA, or (C) AsbB. (D–H) BmcA inhibition against live cultures of microbial strains (D) S. aureus (Newman), (E) B. anthracis (Sterne 34F2), F) MRSA (USA 300), G)
S. flexneri (BS103), or (H) E. coli (MC 1061) in iron-depleted (IDM) or iron-rich (IRM) media. The y-axis represents a comparison of the optical densities of inhibitor- (ODI) and DMSO control-treated (ODC) cultures. Assays were conducted in duplicate due to the current limited availability of the baulamycins.