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. 2013 Aug 7;15(12):2080–2092. doi: 10.1111/cmi.12174

Figure 5.

Figure 5

Assessing the signalling pathways involved in modulation of hβD1 expression in vitro, using gene silencing. hβD1 (A and D), hβD2 (B and E) and IL-8 (C and F) concentrations 24 h after infecting MKN7 (A–C) and AGS (D–F) cells with 60190WT H. pylori. Cells were pre-treated 48 h previously with siRNA duplexes in HiPerfect transfection reagent (HF). siRNA treatments targeted the NFKB1 (NFκBp50), RELA (NFκBp65) and MAPK1 genes. Negative control duplexes were non-silencing, whereas positive control duplexes targeted genes necessary for cell survival. *Significantly different concentration compared with cells treated with negative control siRNA (P < 0.05). Bars represent the mean from three independent experiments and error bars show standard deviations.