FIG. 5.

Histological and immunohistochemical TEVG characterization at explantation. Explants at 8 weeks (top panel) and 24 weeks (middle panel) were assessed with Trichrome (a, e), and immunostaining for vWF (b, f), αSMA (c, g), and elastin (d, h). Grafts at 8 weeks were costained for CD3 (red) and CD11b (green) (i) and at 24 weeks for CD45 (green) (j). (k) scanning electron microscopy (SEM) image of graft lumenal surface at 24 weeks. In all immunostained sections, cell nuclei are counterstained with Hoechst 33342 (blue, except in “h” where nuclear staining is removed for clarity). The symbol * indicates lumenal surface. White scale bar=200 μm, black scale bar=50 μm. Color images available online at www.liebertpub.com/tea