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. 2014 Apr 25;20(11-12):1735–1746. doi: 10.1089/ten.tea.2013.0647

FIG. 5.

FIG. 5.

hAT-MSCs and hBM-MSCs grown in SAGM actively produce surfactant. (A) RT-PCR analysis of hBM-MSCs (A) and hAT-MSCs (B) seeded on an acellular rat lung, then cultured in slices, indicates a progressive increase in SPC expression with time in culture. hBM-MSCs (D) and hAT-MSCs (E) cultured in SAGM for 7 days in a lung slice have visible surfactant droplets in the culture medium adjacent to the lung slice. (C) Enzyme-linked immunosorbent assay (ELISA) of the media samples indicates that at day 3, 5.5 ng/mL of SPC is present in the hAT-MSC lung slice cultures, and 3.3 ng/mL is present in the hBM-MSC cultures. At day 7, the concentration of SPC is 1.1 ng/mL for hAT-MSCs and 0.26 ng/mL for the hBM-MSCs. All ELISA values were normalized to SAGM medium alone (*p<0.05). Bars represent SEM.