FIG. 6.

Substrate coatings influence epithelial marker expression in hBM-MSCs and hAT-MSCs. hBM-MSCs and hAT-MSCs were cultured on various extracellular matrix (ECM) coatings (human ECM, laminin, fibronectin, collagen IV, collagen I, and Matrigel) in SAGM media. After 7 days of culture, the hBM-MSCs (A) and hAT-MSCs (B) were analyzed by RT-PCR for expression of CCSP, pro-SPC, and cytokeratin 5. Both sources of MSCs varied in terms of epithelial marker expression when cultured on different surface coatings. These data indicate that the highest expression of SPC is found in MSCs grown in human ECM compared with MSCs grown on other surface coatings. Also evident from these experiments were differences in cell morphology between hBM-MSCs (C) and hAT-MSCs (D) grown on human ECM.