Table IV.
Effect of protocol modification on initial (before Percoll) and final (after Percoll) cell yield and viability and overall success.
| Factor | Original protocol | New protocol | Unadjusted P valuea |
Adjusted P valueb |
|---|---|---|---|---|
| Initial yield/g tissue, ×106 | 105.1 (28.2–400.8) | 125.0 (41.5–277.6) | 0.775 | 0.872 |
| Initial viability | 10.0% (0.0–31.3%) | 25.0% (14.5–56.3%) | 0.007c | 0.015c |
| Final cell yield, ×106 | 29.0 (12.4–104) | 42.5 (15.9–119.4) | 0.478 | 0.236 |
| Final yield cells/g tissue, ×104 | 27.5 (18.9–134.1) | 48.5 (16.2–114.4) | 0.654 | 0.289 |
| Final viability | 48.0% (0.0–80.0%) | 70.0% (23.2–83.1%) | 0.043c | 0.063 |
| Final viable cell yield/g tissue, ×104 | 7.3 (0.0–20.5) | 28.3 (8.2–70.2) | 0.003c | 0.001c |
| Success | 12 (40.0%) | 21 (70.0%) | 0.037c | 0.014c |
Data displayed as median (quartiles) for continuous variables and n (%) for binary outcomes.
a
P value from Mann-Whitney or Fisher's exact test for continuous and binary outcomes, respectively.
b
P value from a general linear model or binary logistic regression, for continuous and binary outcomes, respectively, after adjustment for the effect of the time delay.
c
Significant at P < 0.05.