. Author manuscript; available in PMC: 2014 May 21.

Published in final edited form as: Hum Pathol. 2012 Apr 26;43(12):2129–2137. doi: 10.1016/j.humpath.2012.01.009

Table 1.

Summary of antibodies used for immunohistochemical analysis

	Vendor	Clone	Pretreatment	Dilution
PTEN	Cell Signaling (Beverly, MA)	D4.3	EDTA, 45 min	1:100
c-MYC	Epitomics (Burlingame, CA)	Y69	EDTA, 45 min	1:300
p27	Transduction Lab (Sparks, MD)	57	Citrate, 25 min	1:4000
phos-AKT ^a	Cell Signaling	736E11	EDTA, 45 min	1:50
phos-S6 ^b	Cell Signaling	Polyclonal	EDTA, 45 min	1:200
4EBP-1	ProSci (San Diego, CA)	Polyclonal	Citrate, 25 min	1:250
HIF-1α	Novus Biologicals (Littleton, CO)	NB100-123	Heat (oven) at 62°C, 60 min	1:1600

Phosphorylation site at Ser473.

Phosphorylation site at Ser235/236.