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. Author manuscript; available in PMC: 2014 May 21.
Published in final edited form as: Methods. 2012 Jul 7;58(3):243–254. doi: 10.1016/j.ymeth.2012.06.019

Figure 3.

Figure 3

ChIP analysis for Satb1 binding sites in the TH2 cytokine locus. This analysis revealed nine Satb1-binding sequences within the 200 kb TH2 locus (SBS-C1-C9). In addition, two other regulatory sequences (CNS-1 and CNS-2) were bound to Satb1. a, Schematic representation of the TH2 cytokine locus; b, Semiquantitative PCR; and c, Real-time PCR analyses of cross-linked chromatin from resting and activated D10.G4.1 cells purified by urea-gradient centrifugation, digested with Sau3AI, and precipitated with anti-Satb1.