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3C and ChIP-3C assays to determine higher-order chromatin structure. These assays were applied to determine intrachromosomal interactions at the TH2 cytokine locus upon activation. a, The experimental strategies of ChIP-3C and 3C assays. b, Analysis of 3C and ChIP-3C data. c, Verification that premixing primers from the TH2 cytokine gene locus and the actin locus does not affect the final results of the 3C assay.
