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. Author manuscript; available in PMC: 2014 May 21.
Published in final edited form as: Methods. 2012 Jul 7;58(3):243–254. doi: 10.1016/j.ymeth.2012.06.019

Figure 5.

Figure 5

Chromatin looping involving SBS-C1 and SBS-C2 before and after activation of TH2 cells. a, Schematic representation of TH2 cytokine locus and positions of 20 Sau3AI DNA fragments used in the ChIP assay are shown. Black lines indicate chromatin looping detected in resting cells. Pink lines indicate DNA fragments showing enhanced interactions upon cell activation. Red lines indicate positions brought in close proximity after activation. Relative cross-linking frequencies (blue bars in the histograms) are shown between fragment 2 (SBS-C1) as a fixed reference point (red bar in the histogram) or b, fragment 20 (SBS-C9) as a fixed reference point and c, the other fragments of the locus. The experiments are as described in Figure 2a, b, and c.