FIG. 2.

(a) Microscopy images of U2OS cells transfected with green fluorescent protein (GFP) and cocultured with immortalized fibroblasts (100×, scale bar=250 μm). (b) Effect of coculture with stromal fibroblasts on angiogenic factor secretion by 2D sorted U2OS cocultured with fibroblasts and 2D unsorted U2OS cocultured with fibroblasts. *Statistically different at p<0.05. (c) Effect of coculture with stromal fibroblasts on angiogenic factor secretion by 2D unsorted U2OS cocultured with fibroblasts and 3D unsorted U2OS cocultured with fibroblasts. *Statistically different at p<0.05. (d) Effect of 3D architecture and coculture on IL-8 expression. Color images available online at www.liebertpub.com/tea