HUVEC migration through Transwell® inserts. (a) HUVEC migration through the Transwell inserts for untreated 3D monoculture (left), 3D monoculture treated with 1 μg/mL anti-VEGF-A monoclonal antibody (center), and 3D monoculture treated with 1 μg/mL anti-IL-8 monoclonal antibody (right); (b) 2D monoculture (left), 2D cocultured with fibroblasts (center), and 2D cocultured with fibroblasts treated with 1 μg/mL anti-IL-8 monoclonal antibody (right); and (c) 3D monoculture (left), 3D cocultured with fibroblasts (center), and 3D cocultured with fibroblasts treated with 1 μg/mL anti-IL-8 monoclonal antibody (right) (DAPI, 40×, scale bar=500 μm). The percentage of the total membrane area covered by the migrated HUVECs is given for each condition. Comparisons should only be made horizontally, as different batches of HUVECs were used for each set of experiments. Color images available online at www.liebertpub.com/tea