Table 1.
Strains and plasmids used.
| Strains/Plasmids | Relevant genotype or description | Source |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | F− φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rk−, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA | Invitrogen Corp. |
| S17-1 | λpir; hsdR pro recA; RP4 2-Tc : : Mu-Km : : Tn7, pro, res−, mod+, StrR, TrmR | (Simon et al., 1983) |
| Pseudomonas aeruginosa | ||
| PAO1 | Wild type strain PAO1 | B.H. Holloway |
| ΔsagS | PAO1, ΔsagS (PA2824) | (Petrova & Sauer, 2011) |
| ΔbrlR | PAO1, ΔbrlR (PA4878) | (Liao & Sauer, 2012) |
| ΔPA4843 | PAO1, allelic gene replacement of PA4843 | This study |
| ΔPA4929 | PAO1, allelic gene replacement of PA4929 | This study |
| ΔbfiS | PAO1; PA4197::ISphoA; TetR | (Petrova & Sauer, 2009) |
| Δpsl | PAO1, WFPA60, ΔpslAB | (Ma et al., 2006) |
| Psl-OE | PAO1, WFPA801, pBAD-psl, arabinose inducible expression of psl operon with psl promoter region replaced by araC-PBAD promoter | (Ma et al., 2006) |
| Pel-OE | PAO1, pBAD-pel, arabinose inducible expression of pel operon with pel promoter region replaced by araC-PBAD promoter | (Ma et al., 2006) |
| Plasmids | ||
| pCR2.1-TOPO | TA cloning vector; KmR; AmpR | Invitrogen Corp. |
| pET101D | Vector for directional cloning and high level V5/6XHis fusion protein expression, AmpR | Invitrogen Corp. |
| pRK2013 | Helper plasmid for triparental mating; mob; tra; KmR | (Figurski & Helinski, 1979) |
| pJN105 | Arabinose-inducible gene expression vector; pBRR-1 MCS; araC-PBAD; GmR | (Newman & Fuqua, 1999) |
| pMJT1 | araC-PBAD cassette of pJN105 cloned into pUCP18, AmpR (CarbR) | (Kaneko et al., 2007) |
| pEX18Gm | Allelic replacement suicide vector; pUC18 MCS; oriT+; sacB+; GmR | (Hoang et al., 1998) |
| pMini-CTX-lacZ | attP site-specific integration vector, TetR | (Becher & Schweizer, 2000) |
| pEX18-PA4843 | Vector for allelic gene replacement of PA4843 | This study |
| pMJT-dipA | dipA (PA5017) cloned into pMJT1; AmpR (CarbR) | (Basu Roy et al., 2012) |
| pMJT-rbdA | rbdA (PA0861) cloned into pMJT1; AmpR (CarbR) | This study |
| pMJT-PA4843 | PA4843 cloned into pMJT1; AmpR (CarbR) | (Petrova & Sauer, 2012) |
| pMJT-PA4929 | PA4929 cloned into pMJT1; AmpR (CarbR) | This study |
| pMJT-brlR-His6V5 | C-terminal His6/V5-tagged brlR cloned into pMJT1; AmpR (CarbR) | (30) |
| pJN-PA4843 | PA4843 cloned into pJN105; GmR | (Petrova & Sauer, 2012) |
| pJN-brlR | brlR (PA4843) cloned into pJN105; GmR | (Liao & Sauer, 2012) |
| PbrlR-brlR-His6V5 | pMini-CTX harboring C-terminal His6/V5-tagged brlR under the control of the brlR promoter region (1–120 bp upstream of the brlR start codon) | This study |