Table 1.
Spectral absorbance characteristics for wild type C. nuchalis SWS1 pigments, site-directed mutants, and ancestral pigments
| Pigment | λ max a (nm) | Shift from C. nuchalis wt pigment b (nm) |
|---|---|---|
| GBS1 wt |
402.97 ± 0.22 |
|
| S90C |
363.05 ± 0.05 |
-40 |
| C86S |
403.05 ± 0.12 |
0 |
| C86Fc |
365.72 ± 1.10 |
-37 |
| C86S/S90C |
362.95 ± 0.26 |
-40 |
| Parrot/Passerine Ancestor |
402.93 ± 0.28 |
0 |
| Passerine Ancestor | 404.28 ± 0.28 | 0 |
a λmax values are given as mean ± standard deviation from at least three different measurements of dark absorbance spectra per expression. b λmax shifts from C. nuchalis wild type (wt) pigment are expressed as negative for blue shifts. c λmax of single mutant C86F calculated from fitting difference spectra of dark and acid denatured species.