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. 2013 Dec 11;13:289. doi: 10.1186/1471-2180-13-289

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Copyright © 2013 He et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunofluorescence analysis of TNF-α expression in gut. A: TNF-α expression was stimulated in larvae exposed to TNBS. TNF-α staining (red) and DAPI staining (blue) images were visualized by confocal laser scanning microscopy. Bars: 25 μm. B: TNF-α immunofluorescence levels increased with increasing concentrations of TNBS over time. All error bars represent as mean ± SEM, n=13–16 sections per group, ^aIndicates a significant difference (p<0.05) between TNBS-exposed group (25 μg/ml) and the control, ^bIndicates a significant difference (p<0.05) between TNBS-exposed group (50 μg/ml) and the control, ^cIndicates a significant difference (p<0.05) between TNBS-exposed group (75 μg/ml) and the control, ^dIndicates a significant difference (p<0.05) between control groups at 6 dpf and 4 dpf, ^eIndicates a significant difference (p<0.05) between control groups at 8 dpf and 4 dpf.