Table 1.

Primer sequence for the amplification of pfdhfr and pfdhps

PCR reaction		Primer pair sequence	Expected size	Reference
pfdhfr	Nest 1	Pfdhfr_D1 5′ TTTATATTTTCTCCTTTTTA 3′	718 bp	[9]
		Pfdhfr_D2 5′ CATTTTATTATTCGTTTTCT 3′
	Nest 2	Pfdhfr_M1 5′ TTTATGATGGAACAAGTCTGC 3′	648 bp
		Pfdhfr_M5 5′ AGTATATACATCGCTAACAGA 3′
pfdhps	Nest 1	Pfdhps_N185 5′ TGATACCCGAATATAAGCATAATG 3′	1031 bp	[10]
		Pfdhps_N218 5′ ATAATAGCTGTAGGAAGCAATTG 3′
	Nest 2	Pfdhps_Rc 5′ GGTATTTTTGTTGAACCTAAACG 3′	728 bp
		Pfdhfr_D2 5′ ATCCAATTGTGTGATTTGTCCAC 3′