Table 1.

Strains and plasmids used in this study

Strain or plasmid	Relevant genotype	Reference or source
Strains
NCTC8325	Wild-type	NARSAa
RN4220	8325-4 r- initial recipient for modification of plasmids which are introduced into S. aureus from E. coli	NARSA
ΔairSR	8325 airSR::ermB	This study
CairSR	8325 airSR::ermB pLIairSR	This study
DH5α	Clone host strain, supE44 ΔlacU169 (φ80dlacZΔM15) hsdR17 recA1 endA1gyrA96 thi-1 relA1	TransGen
BL21 (DE3)	Express strain, F-ompT hsdSB (rB- mB-) gal dcm(DE3)	TransGen
Plasmids
pEasy-blunt simple	Clone vector, Kanr Apr b	TransGen
pET28a(+)	Expression vector with a hexahistidine tag, Kanr	Novagen
pEairR	pET28a(+) with the airR coding sequence, Kanr	This study
pEairS	pET28a(+) with the airS coding sequence, Kanr	This study
pEC1	pUC18 derivative, source of the ermB gene, Apr	Bruckner
pBT2	Shuttle vector, temperature sensitive, Apr Cmr	Bruckner
pBTairSR	pBT2 containing upstream and downstream fragments of airSR and ermB gene, for airSR mutagenesis, Apr Cmr Emr	This study
pLI50	Shuttle cloning vector, Apr Cmr	Addgene
pLIairSR	pLI50 with airSR ORF and its promoter, Apr Cmr	This study

^aNARSA, Network on Antimicrobial Resistance in Staphylococcus aureus;

^bKan^r, kanamycin-resistant; Ap^r, ampicillin-resistant; Cm^r, chloramphenicol-resistant; Em^r, erythromycin-resistant.