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. 2013 Dec 10;13:286. doi: 10.1186/1471-2180-13-286

Table 2.

Primers used in this study

Primer name Oligonucleotide (5′-3′) a Application
up-airSR-f
CCGgaattcTACATCTTGTGCCTTAG
airSR deletion
up-airSR-r
ATTTGAGatcgatAATGTTCAG
airSR deletion
down-airSR-f
CGATTTAAGTggtaccGTTGCATGATGTG
airSR deletion
down-airSR-r
CGCggatccCCTTAAGTTGTTGGAA
airSR deletion
Em-f
CGGatcgatGATACAAATTCCCCGTAGGC
airSR deletion
Em-r
CGGggtaccGAAATAGATTTAAAAATTTCGC
airSR deletion
c-airSR-f
CGCggatccATCGTCGCCAGTATG
ΔairS complementation
c-airSR-r
CCGgaattcTGAAGCGAAAGTAAATG
ΔairS complementation
e-airR-f
GGAATTCcatatgAACAAAGTAATATT
expression of AirR
e-airR-r
CCGctcgagAATCAACTTATTTTCCA
expression of AirR
e-airS-f
GGGAATTCcatatgATGGAACAAAGGACGCGACTAG
expression of AirS
e-airS-r
CCGctcgagCTATTTTATAGGAATTGTGAATTG
expression of AirS
RTQ-cap5B-f
GCTTATTGGTTACTTCTGA
real-time RT PCR
RTQ-cap5B-r
GTTGGCTTACGCATATC
real-time RT PCR
RTQ-cap5D-f
ATATGCCAGTGTGAGTGA
real-time RT PCR
RTQ-cap5D-r
CGGTCTATTGCCTGTAAC
real-time RT PCR
RTQ-lytM-f
CATTCGTAGATGCTCAAGGA
real-time RT PCR
RTQ-lytM-r
CTCGCTGTGTAGTCATTGT
real-time RT PCR
RTQ-640-f
TGATGGGACAGGAGT
real-time RT PCR
RTQ-640-r
TATTGTGCCGCTTCT
real-time RT PCR
RTQ-953-f
GTCATTGAGCACGATTTATT
real-time RT PCR
RTQ-953-r
TCTGGGCGGCTGTAA
real-time RT PCR
RTQ-pbp1-f
AGTCAGCGACCAACATT
real-time RT PCR
RTQ-pbp1-r
AAGCACCTTCTTGAATAGC
real-time RT PCR
RTQ-murD-f
TTCAGGAATAGAGCATAGA
real-time RT PCR
RTQ-murD-r
AACCACCACATAACCAA
real-time RT PCR
RTQ-1148-f
GCCGAAGTGACATAC
real-time RT PCR
RTQ-1148-r
AAGCACCGACTGATA
real-time RT PCR
RTQ-ddl-f
TAGGGTCAAGTGTAGGT
real-time RT PCR
RTQ-ddl-r
GTCGCTTCAGGATAG
real-time RT PCR
RTQ-pta-f
AAAGCGCCAGGTGCTAAATTAC
real-time RT PCR
RTQ-pta-r
CTGGACCAACTGCATCATATCC
real-time RT PCR
p-cap5A-f
TCATCTAACTCACCTGAAATTACAAAA
EMSA
p-cap5A-r
TTTCCATTATTTACCTCCCTTAAAAA
EMSA
p-ddl-f
CAAACTCCTTTTATACTC
EMSA
p-ddl-r
GTCATTTCGTTTTCCT
EMSA
p-pbp1-f
GATTCAATAGAACAAGCGATT
EMSA
p-pbp1-r
AGCTACACGTAATTTCGCGCTT
EMSA
p-lytM-f
GAATCGCGAACATGGACGAA
EMSA
p-lytM-r GCAATCGCTGCTGCTGTTAA EMSA

aThe sequences in lowercase letters refer to the restriction endonuclease recognition sites.