Table 2.
Primers used in this study
| Primer name | Oligonucleotide (5′-3′) a | Application |
|---|---|---|
| up-airSR-f |
CCGgaattcTACATCTTGTGCCTTAG |
airSR deletion |
| up-airSR-r |
ATTTGAGatcgatAATGTTCAG |
airSR deletion |
| down-airSR-f |
CGATTTAAGTggtaccGTTGCATGATGTG |
airSR deletion |
| down-airSR-r |
CGCggatccCCTTAAGTTGTTGGAA |
airSR deletion |
| Em-f |
CGGatcgatGATACAAATTCCCCGTAGGC |
airSR deletion |
| Em-r |
CGGggtaccGAAATAGATTTAAAAATTTCGC |
airSR deletion |
| c-airSR-f |
CGCggatccATCGTCGCCAGTATG |
ΔairS complementation |
| c-airSR-r |
CCGgaattcTGAAGCGAAAGTAAATG |
ΔairS complementation |
| e-airR-f |
GGAATTCcatatgAACAAAGTAATATT |
expression of AirR |
| e-airR-r |
CCGctcgagAATCAACTTATTTTCCA |
expression of AirR |
| e-airS-f |
GGGAATTCcatatgATGGAACAAAGGACGCGACTAG |
expression of AirS |
| e-airS-r |
CCGctcgagCTATTTTATAGGAATTGTGAATTG |
expression of AirS |
| RTQ-cap5B-f |
GCTTATTGGTTACTTCTGA |
real-time RT PCR |
| RTQ-cap5B-r |
GTTGGCTTACGCATATC |
real-time RT PCR |
| RTQ-cap5D-f |
ATATGCCAGTGTGAGTGA |
real-time RT PCR |
| RTQ-cap5D-r |
CGGTCTATTGCCTGTAAC |
real-time RT PCR |
| RTQ-lytM-f |
CATTCGTAGATGCTCAAGGA |
real-time RT PCR |
| RTQ-lytM-r |
CTCGCTGTGTAGTCATTGT |
real-time RT PCR |
| RTQ-640-f |
TGATGGGACAGGAGT |
real-time RT PCR |
| RTQ-640-r |
TATTGTGCCGCTTCT |
real-time RT PCR |
| RTQ-953-f |
GTCATTGAGCACGATTTATT |
real-time RT PCR |
| RTQ-953-r |
TCTGGGCGGCTGTAA |
real-time RT PCR |
| RTQ-pbp1-f |
AGTCAGCGACCAACATT |
real-time RT PCR |
| RTQ-pbp1-r |
AAGCACCTTCTTGAATAGC |
real-time RT PCR |
| RTQ-murD-f |
TTCAGGAATAGAGCATAGA |
real-time RT PCR |
| RTQ-murD-r |
AACCACCACATAACCAA |
real-time RT PCR |
| RTQ-1148-f |
GCCGAAGTGACATAC |
real-time RT PCR |
| RTQ-1148-r |
AAGCACCGACTGATA |
real-time RT PCR |
| RTQ-ddl-f |
TAGGGTCAAGTGTAGGT |
real-time RT PCR |
| RTQ-ddl-r |
GTCGCTTCAGGATAG |
real-time RT PCR |
| RTQ-pta-f |
AAAGCGCCAGGTGCTAAATTAC |
real-time RT PCR |
| RTQ-pta-r |
CTGGACCAACTGCATCATATCC |
real-time RT PCR |
| p-cap5A-f |
TCATCTAACTCACCTGAAATTACAAAA |
EMSA |
| p-cap5A-r |
TTTCCATTATTTACCTCCCTTAAAAA |
EMSA |
| p-ddl-f |
CAAACTCCTTTTATACTC |
EMSA |
| p-ddl-r |
GTCATTTCGTTTTCCT |
EMSA |
| p-pbp1-f |
GATTCAATAGAACAAGCGATT |
EMSA |
| p-pbp1-r |
AGCTACACGTAATTTCGCGCTT |
EMSA |
| p-lytM-f |
GAATCGCGAACATGGACGAA |
EMSA |
| p-lytM-r | GCAATCGCTGCTGCTGTTAA | EMSA |
aThe sequences in lowercase letters refer to the restriction endonuclease recognition sites.