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. 2013 Nov 4;6:317. doi: 10.1186/1756-3305-6-317

Figure 1.

Figure 1

TLR2 ligands but not LPS augment the secretion of IL-12 cytokines by human DCs incubated in the presence of G. duodenalis. Immature DCs were incubated with (A) LPS (1 μg/ml), (B) Pam3CSK4 (100 ng/ml), or (C) Pam2CSK4 (100 ng/ml) in the presence or absence of G. duodenalis lysate (equivalent to five trophozoites/cell). Two days later, cell-free supernatants were collected, stored at -80°C, and the cytokine concentrations determined by ELISA. Median plus 25% and 75% percentiles for cells from ten donors. Dotted horizontal lines indicate the level of detection of the ELISA assays (5 pg/ml).