Table 1.
DC activation markers expressed by human DCs incubated in the presence of ligands for TLR4, TLR2/TLR1, or TLR2/TLR6, and in the presence or absence of G. duodenalis lysate
| Molecule | LPS | LPS + Gd | Pam3CSK | Pam3CSK + Gd | Pam2CSK4 | Pam2CSK4 + Gd |
|---|---|---|---|---|---|---|
| CD25 |
84 [73, 90]# |
82 [66, 88]* |
8 [3, 21] |
20 [7, 30]* |
10 [3, 27] |
21 [9, 35]* |
| CD83 |
90 [85, 95] |
84 [80, 93]* |
18 [12, 51] |
35 [22, 59]* |
23 [15, 51] |
39 [24, 63]* |
| CD86 |
98 [97, 99] |
96 [93, 97]* |
51 [48, 61] |
67 [59, 79]* |
56 [48, 71] |
70 [61, 86]* |
| HLA-DR | 99 [98, 99] | 97 [95, 98]* | 88 [84, 97] | 90 [86, 97] | 88 [84, 96] | 92 [84, 97] |
#Median percentage of positive cells (25% and 75% percentiles given in square brackets) obtained with cells from ten donors, except for CD86, which could be analyzed for eight donors only (data of isotype controls were subtracted).
*p < 0.05 compared to DCs incubated in the presence of LPS, Pam3CSK, or Pam2CSK4 but in the absence of G. duodenalis (Wilcoxon matched-pairs signed rank test).