Figure 3. Trophoblast cells restrain maternal monocytes migration depending on the pathological stimuli.

Migration assays were performed in transwell system. Condition media from Swan-71 cells cultured in the absence or presence of LPS (10 µg/ml), PGN (10 µg/ml), or poly [I:C] (10 µg/ml) was used in the lower compartment as attractant and 8 µm-pore inserts were added containing the isolated monocytes (upper compartment). After 24 and 48 hours, cells were recovered from the lower and upper compartments and the frequency of CD14+ cells were quantified by FACS analysis. As a positive control, we used 20% human AB serum. Results are expressed as percentage of migration (mean ± SEM) from 4 migration assays using different fertile women (*p<0.05, Friedman test followed by Dunn’s post-test).