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. 2014 May 21;9(5):e97147. doi: 10.1371/journal.pone.0097147

Table 2. Description of the PCR amplification programs.

CCL5, CCL2, CXCL8, GapDH CCR1 CCR3, CCR5 TLR2, TLR3, TLR4
Initial denaturalization 95°C, 5 min 95°C, 5 min 95°C, 5 min
Cycles 35 35 35
- Denaturalization 95°C, 20s 95°C, 1 min 95°C, 20s
- Primer annealing 62°C, 20s 55°C, 1 min 55°C, 20s
- Elongation 72°C, 20s 72°C, 1 min 72°C, 20s
Final elongation 72°C, 10 min 72°C, 15 min 72°C, 10 min