Table 2. Description of the PCR amplification programs.
CCL5, CCL2, CXCL8, GapDH | CCR1 CCR3, CCR5 | TLR2, TLR3, TLR4 | |
Initial denaturalization | 95°C, 5 min | 95°C, 5 min | 95°C, 5 min |
Cycles | 35 | 35 | 35 |
- Denaturalization | 95°C, 20s | 95°C, 1 min | 95°C, 20s |
- Primer annealing | 62°C, 20s | 55°C, 1 min | 55°C, 20s |
- Elongation | 72°C, 20s | 72°C, 1 min | 72°C, 20s |
Final elongation | 72°C, 10 min | 72°C, 15 min | 72°C, 10 min |