Figure 6.

Competition in PHB binding between His₆-tag PhaP4 and His₆-tag PhaR. The amount of crude extract was compared to controls and fixed to contain His₆-tag PhaR equivalent to 0.094% (w/v) PHB in each of the tubes, and then various amounts of extract containing His₆-Tag PhaP4 were added and incubated to allow formation of PHB/protein complexes. The complexes were spun down and subjected to the immunoblot analysis described in Figure 5. Lane 1 contains His₆-tag PhaR alone and no His₆-tag PhaP4. Concentrations of His₆-tag PhaR and His₆-tag PhaP are controlled in the ratios of 4:1 (lane 2), 4:2 (lane 3), 4:4 (lane 4), 4:8 (lane 5), and 4:16 (lane 5). One set of representative data, from three independent experiments with similar results, is shown.