Table 1.
Bacterial strains and plasmids
| Strains and plasmids | Relevant genotypes and derivation | Source and reference |
|---|---|---|
|
B. japonicum | ||
| USDA110 |
|
24 |
|
E. coli | ||
| DH5a |
supE44, DlacU169, hsdR17, recA1, endA1, gyrA96, thi-1, relA1 |
Laboratory stocks |
| BL21 (DE3) |
F-ompT hsdSb (rb-mb-) gal dcm (DE3) |
Laboratory stocks |
| Plasmids | ||
| pET-28b |
Protein expression vector, kanamycin resistant |
Takara Bio |
| pETPhaP1 |
pET28b carrying phaP1 |
This work |
| pETPhaP2 |
pET28b carrying phaP2 |
This work |
| pETPhaP3 |
pET28b carrying phaP3 |
This work |
| pETPhaR |
pET28b carrying phaR |
This work |
| pColdII |
Protein expression vector, ampicillin resistant |
Takara Bio |
| pColdPhaP4 | pColdII carrying phaP4 | This work |