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. 2013 Dec 11;13:290. doi: 10.1186/1471-2180-13-290

Table 2.

Oligonucleotide primers

Oligonucleotide primers Sequence (designed restriction sites are underlined)
PhaP1 NdeI
5′-GGAATTCCATATGATGTTCAAGGTTGAAGACTT-3′
PhaP1 XhoI
5′-CCGCTCGAGTTAGTTGGCGGCCGGGGTGA-3′
PhaP2 NdeI
5′-GGAATTCCATATGGTGAGTGATGCCGATATGAA-3′
PhaP2 XhoI
5′-CCGCTCGAGTCAAGCAGCCCTATGCAGAA-3′
PhaP3 NdeI
5′-GGAATTCCATATGATGATCGAACCGAAAATCGA-3′
PhaP3 XhoI
5′-CCGCTCGAGTCAGGATTTGCCCTTGCCCG-3′
PhaP4 NdeI
5′-GGAATTCCATATGATGACAGGTGCGACTGATCC-3′
PhaP4 HindIII
5′-CCCAAGCTTTCAGGCGGCGGGCGGGTAGA-3′
PhaR NdeI
5′-GGAATTCCATATGGCGAAATCAGACCAACCCAC-3′
PhaR HindIII
5′-CCCAAGCTTCTACTCTTCCTTCTTCGACA-3′
RTphbA1-F
5′-CATCGCCGTCAACAAGGA-3′
RTphbA1-R
5′-CCGCTTCTGCATCTCGAAC-3′
RTphbA2-F
5′-AAGAAGGCCGGCTGGAA-3′
RTphbA2-R
5′-CCATTGACGTTGACCTTGGA-3′
RTphbB1-F
5′-TCGAACTACGACGCCTGTG-3′
RTphbB1-R
5′-ATGCCGTCCTTGGTGATG-3′
RTphbB2-F
5′-CCGAAGGCGTGAAGAAGGT-3′
RTphbB2-R
5′-GAACAGCGAGCCGAGATTG-3′
RTphbC1-F
5′-GCTCTGGGAAAACATCTGGAAC-3′
RTphbC1-R
5′-TTGGTGATGGTGCGGAAA-3′
RTphbC2-F
5′-GGACGACTACGTTGAGGATGG-3′
RTphbC2-R
5′-AATGGCGAGTGCGGATG-3′
RTphbC3-F
5′-ATGACCGCGTCGAACCA-3′
RTphbC3-R
5′-GGCACCTTGACCTTGGAGA-3′
RTphbC4-F
5′-GGCGAAGACAGGCAAACA-3′
RTphbC4-R
5′-CTCCATCCATCCGAACCA-3′
RTphbC5-F
5′-CCGCAAAATTCCCTGGTC-3′
RTphbC5-R
5′-CATCCCTGTCCTTCGCATC-3′
RTphaZ1-F
5′-CCGAAGCAACGCACACA-3′
RTphaZ1-R
5′-ATCCTCGGCACGATTTCC-3′
RTphaZ2-F
5′-GGCACATCAAGCAGCACA-3′
RTphaZ2-R
5′-AGATCCATCACCGCGAAA-3′
RTphaP1-F
5′-ACGGCGACTACACCAAGAAG-3′
RTphaP1-R
5′-GAAGGTCTCGTAGGCGGAAC-3′
RTphaP2-F
5′-TCGCTTTTACCGAGCAGAAC-3′
RTphaP2-R
5′-GTGAACTGGCTACGCAGGA-3′
RTphaP3-F
5′-ATTACGGCACCAAGGTCATC-3′
RTphaP3-R
5′-GTGGAGAGGTTCACGAGGTC-3′
RTphaP4-F
5′-GTGCGACTGATCCATTCTCC-3′
RTphaP4-R
5′-GTCCTTGAACTTGGCGTAGC-3′
RTphaR-F
5′-CTTCGAGCAGGAGAACAAGG-3′
RTphaR-R
5′-GATATTTCGGCACCACCATC-3′
RTsigA-F
5′-CAGGCGAAGGACAAGGAAAA-3′
RTsigA-R 5′-CGTCGGACAGATCGAGCAA-3′