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. 2014 Apr 9;14(4):6463–6473. doi: 10.3390/s140406463

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 6. — Detection of lactonase activity using CV026 overlay. Yeast suspensions (B2 and B3) were incubated with 3-oxo-C6-HSL for 0 h and 24 h as indicated on the left of figure. Positive QQ activity can be seen as the abolishment of purple pigments after 24 h of incubation. B. cereus represents the positive control while E. coli TOP10 and PBS buffer acted as negative controls. The purple pigments with the addition of hydrochloric acid (HCl) suggested re-lactonisation of the digested AHL indicated that both the isolates produced lactonase in the presence of 3-oxo-C6-HSL.